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Crystal growth: Tips and Tricks: Difference between revisions
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→Reducing the mosaicity
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==Improving crystals== | ==Improving crystals== | ||
===Reducing the mosaicity=== | ===Reducing the mosaicity=== | ||
* incubate your crystal longer in your cryo/stabilization solution. This helps sometimes to lower | * incubate your crystal longer in your cryo/stabilization solution. This helps sometimes to lower mosaicity. Of course, you can also try co-crystallization with glycerol (2%, 5% or 10%). | ||
mosaicity. Of course, you can also try co-crystallization with glycerol (2%, 5% or 10%). | |||
* the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | * the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | ||
* look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | * look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | ||
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* use a synchrotron beam (as it is less divergent than the usual home source, and the apparent mosaicity is the sum of the crystal mosaicity and the crossfire) | * use a synchrotron beam (as it is less divergent than the usual home source, and the apparent mosaicity is the sum of the crystal mosaicity and the crossfire) | ||
* try smaller crystals (often growth faults lead to end of crystal growth) | * try smaller crystals (often growth faults lead to end of crystal growth) | ||
* shoot at parts of the | * shoot at parts of the crystal to find if there are good and bad parts (this requires a small beam) | ||