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Crystal growth: Tips and Tricks: Difference between revisions
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→Reducing the mosaicity
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==Improving crystals== | ==Improving crystals== | ||
===Reducing the mosaicity=== | ===Reducing the mosaicity=== | ||
* incubate your crystal longer in your cryo/stabilization solution. This helps sometimes | * incubate your crystal longer in your cryo/stabilization solution. This helps sometimes. | ||
* try co-crystallization with glycerol (2%, 5% or 10%). | |||
* the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | * the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | ||
* look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | * look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | ||