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#* [http://www.emeraldbiosystems.com Emerald Biosystems] | #* [http://www.emeraldbiosystems.com Emerald Biosystems] | ||
#* [http://www.jenabioscience.com/cms/en/1/browse/632_screens.html Jena Bioscience] | #* [http://www.jenabioscience.com/cms/en/1/browse/632_screens.html Jena Bioscience] | ||
#* [[Crystallization screens and methods | #* [http://www.moleculardimensions.com Molecular Dimensions] | ||
#* See also [[Crystallization screens and methods]] | |||
# [[Modifying_the_protein_to_crystallize_better | Modify your protein. ]] | # [[Modifying_the_protein_to_crystallize_better | Modify your protein. ]] | ||
==Improving crystals== | ==Improving crystals== | ||
===Reducing the mosaicity=== | ===Reducing the mosaicity=== | ||
Things to try | |||
Things to try concerning crystallization - see also [[Improving crystal quality]]. | |||
For cryo question see [[cryo]] and [[Data collection: Tips and Tricks]]. | |||
* change precipitant, pH, temperature; try additives | |||
* try co-crystallization with glycerol (2%, 5% or 10%). | |||
* incubate your crystal longer in your cryo/stabilization solution. | |||
* optimize the cryo conditions | |||
* anneal (freeze/thaw) the crystal - '''DISCLAIMER''': your mosaicity can go up as well as down with annealing | |||
* the mosaicity may be anisotropic, so try to orient the crystal in the loop such that you can shoot along a different axis | |||
* look at Elspeth Garman's papers, and/or check her talks at RapiData workshops (anybody with a link??) | |||
==References== | |||
* J.W. Pflugrath (2004) Macromolecular cryocrystallography—methods for cooling and mounting protein crystals at cryogenic temperatures. ''Methods'' '''34''', 415-423 http://dx.doi.org/10.1016/j.ymeth.2004.03.032 | |||
* papers listed at http://www.px.nsls.bnl.gov/courses/papers/ZD_EG_papers.html | |||
* H.D. Bellamy, E.H. Snell, J. Lovelace, M. Pokross and G.E.O. Borgstahl (2000) The high-mosaicity illusion: revealing the true physical characteristics of macromolecular crystals. ''Acta Cryst.'' '''D56''', 986–995 |
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